Fundamentals of Genetic Engineering

1.

Subject title

Fundamentals of Genetic Engineering

Основи на генетско инженерство

2.

Code

m23_s_002

3.

Study program

Cloud Computing, Data science in computer science and engineering, IT management, Security, Cryptography and Coding, Еducation with ICT, Eco-informatics, Inteligent Systems, Internet Technologies and cyber security, Computer Science, Statistics and Data Analytics, Software for embedded systems, Software Engineering, Cloud Computing, Security, Cryptography and Coding, Statistics and Data Analytics, IT management, Software Engineering, Bioinformatics, Bioinformatics,

4.

Organizer of the study program (unit, institute, department, division)

Faculty of Information Sciences and Computer Engineering

5.

Study cycle (first, second, third)

Втор циклус

6.

Academic year / semester

5 / Летен

7. Number of ECTS credits

6.0

8.

Instructor

виз. проф. д-р Сашо Панов проф. д-р Невена Ацковска

9.

Prerequisites for enrollment

10.

Subject goals and competencies:

This course presents the basics of genetic engineering. Typically the methods used in genetic engineering are considered very close to the biomedical sciences. However, in the last decade it has been shown that more and more researchers from classical engineering disciplines are focused on generating desired characteristics using biological principles. After successful completion of this course, the student will be able to understand the ways in which genes are separated and modified, in nature and in laboratory conditions. Students should acquire extensive knowledge of recombinant DNA technology (genetic engineering) and their applications.

11.

Subject content:

Principles of genetic engineering; methodology for manipulating nucleic acids. Prokaryotic and eukaryotic host cells; vectors (plasmids, bacteriophages, animal viruses, hybrid and advanced vectors). Techniques for introduction of exogenous DNA into cells. Restriction digests and genome mapping. Ligation-linking in cloning. Cloning strategies; gene libraries; PCR as an alternative to gene cloning; cloning of complementary DNA. DNA sequencing and mutagenesis in vitro. Genome editing with the CRISP/CASP system. Genetic manipulation in experimental animals and model organisms in genetics. Application of genetic engineering in biology, medicine, pharmacy and others. Ethics and genetic engineering

12.

Learning methods:

Предавања поддржани со презентации преку слајдови, интерактивни предавања, вежби (користење на опрема и софтверски пакети), тимска работа, пример случаи, поканети гости предавачи, самостојна изработка и одбрана на проектна задача и семинарска работа, учење во електронско опкружување (форуми, консултации).

13.

Total available time fund

6.0 ECTS x 30 hours = 180 hours

14.

Time distribution

60 + 0 + 45 + 45 + 30 = 180 hours

15.

Forms of teaching activities

15.1.

Lectures - theoretical teaching

60 hours

15.2.

Exercises (laboratory, classroom), seminars, team work

0 hours

16.

Other forms of activities

16.1.

Project tasks

45 hours

16.2.

Independent tasks

45 hours

16.3.

Homework

30 hours

17.

Grading method

17.1.

Tests

15 points

17.2.

Seminar work / project (presentation: written and oral)

45 points

17.3.

Activities and learning

15 points

17.4.

Final exam

0 points

18.

Grading criteria (points / grade)

up to 50 points

5 (five) (F)

from 51 to 60 points

6 (six) (E)

from 61 to 70 points

7 (seven) (D)

from 71 to 80 points

8 (eight) (C)

from 81 to 90 points

9 (nine) (B)

from 91 to 100 points

10 (ten) (A)

19.

Condition for signature and taking final exam

реализирани активности 15

20.

Language of instruction

македонски или англиски

21.

Quality assurance method

механизам на интерна евалуација и анкети

22.

Literature

22.1.

Mandatory literature

22.2.

Additional literature